Publicaciones

  1. García-Hernández S, Rubio L, Rivera-Moreno M, Pérez-Sancho J, Morello-López J, Esteban Del Valle A, Benítez-Fuente F, Beuzón CR, Macho AP, Ruiz-López N, Albert A, Botella MA. Functional and Structural Analysis Reveals Distinct Biological Roles of Plant Synaptotagmins in Response to Environmental Stress. Plant Cell Environ. 2024 Sep 10. doi: 10.1111/pce.15125. Epub ahead of print.
  2. López-Pagán N, Rufián JS, Ruiz-Albert J, Beuzón CR. Dual-Fluorescence Chromosome-Located Labeling System for Accurate In Vivo Single-Cell Gene Expression Analysis in Pseudomonas syringae. Methods Mol Biol. 2024;2751:95-114. doi: 10.1007/978-1-0716-3617-6_7. PMID: 38265712.
  3. Rufián JS, Rueda-Blanco J, Beuzón CR, Ruiz-Albert J. Suppression of NLR-mediated plant immune detection by bacterial pathogens. J Exp Bot. 2023 Jul 10:erad246. doi: 10.1093/jxb/erad246. PMID: 37429579.
  4. López-Márquez D, Del-Espino A, Ruiz-Albert J, Bejarano ER, Brodersen P, Beuzón CR. Regulation of plant immunity via small RNA-mediated control of NLR expression. J Exp Bot. 2023 Jul 14:erad268. doi: 10.1093/jxb/erad268. PMID: 37449766.
  5. Rufián JS, López-Pagán N, Ruiz-Albert J, Beuzón CR. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J Vis Exp. 2022 Oct 6;(188). doi: 10.3791/64614. PMID: 36282707.
  6. Rufián, JS; Ruiz-Albert, J; Beuzón, CR (2022). Fluorescently labeled Pseudomonas syringae DC3000 and 1449b wild-type strains constitutively expressing either eGFP, eCFP, or dsRED. microPublication Biology. 10.17912/micropub.biology.000595.
  7. López-Márquez D, Del-Espino Á, López-Pagán N, Rodríguez-Negrete EA, Rubio-Somoza I, Ruiz-Albert J, Bejarano ER, Beuzón CR. miR825-5p targets the TIR-NBS-LRR gene MIST1 and down-regulates basal immunity against Pseudomonas syringae in Arabidopsis. J Exp Bot. 2021 Oct 26;72(20):7316-7334. doi: 10.1093/jxb/erab354. PMID: 34329403; PMCID: PMC8547151.
  8. Rufián JS, Elmore JM, Bejarano ER, Beuzon CR, Coaker GL. ER Bodies Are Induced by Pseudomonas syringae and Negatively Regulate Immunity. Mol Plant Microbe Interact. 2021 Sep;34(9):1001-1009. doi: 10.1094/MPMI-11-20-0330-SC. Epub 2021 Sep 29. PMID: 34110257; PMCID: PMC8635791.
  9. Rufián JS, Rueda-Blanco J, López-Márquez D, Macho AP, Beuzón CR, Ruiz-Albert J. The bacterial effector HopZ1a acetylates MKK7 to suppress plant immunity. New Phytol. 2021 Aug;231(3):1138-1156. doi: 10.1111/nph.17442. Epub 2021 Jun 5 PMID: 33960430.
  10. Jiménez-Ruiz J, Ramírez-Tejero JA, Fernández-Pozo N, Leyva-Pérez MO, Yan H, Rosa R, Belaj A, Montes E, Rodríguez-Ariza MO, Navarro F, Barroso JB, Beuzón CR, Valpuesta V, Bombarely A, Luque F. Transposon activation is a major driver in the genome evolution of cultivated olive trees (Olea europaea L.). Plant Genome. 2020 Mar;13(1):e20010. doi: 10.1002/tpg2.20010. Epub 2020 Mar 27. PMID: 33016633.
  11. Zarkani AA, López-Pagán N, Grimm M, Sánchez-Romero MA, Ruiz-Albert J, Beuzón CR, Schikora A. Salmonella Heterogeneously Expresses Flagellin during Colonization of Plants. Microorganisms. 2020 May 29;8(6):815. doi: 10.3390/microorganisms8060815. PMID: 32485895; PMCID: PMC7355505.
  12. Arce-Leal ÁP, Bautista R, Rodríguez-Negrete EA, Manzanilla-Ramírez MÁ, Velázquez-Monreal JJ, Santos-Cervantes ME, Méndez-Lozano J, Beuzón CR, Bejarano ER, Castillo AG, Claros MG, Leyva-López NE. Gene Expression Profile of Mexican Lime (Citrus aurantifolia) Trees in Response to Huanglongbing Disease caused by Candidatus Liberibacter asiaticus. Microorganisms. 2020 Apr 7;8(4):528. doi: 10.3390/microorganisms8040528. PMID: 32272632; PMCID: PMC7232340.
  13. López-Márquez D, Del-Espino Á, Bejarano ER, Beuzón CR, Ruiz-Albert J. Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis. Plant Methods. 2020 Mar 20;16:41. doi: 10.1186/s13007-020-00581-w. PMID: 32206081; PMCID: PMC7082952.
  14. Calvo-Polanco M, Ruiz-Lozano JM, Azcón R, Molina S, Beuzon CR, García JL, Cantos M, Aroca R. Phenotypic and molecular traits determine the tolerance of olive trees to drought stress. Plant Physiol Biochem. 2019 Jun;139:521-527. doi: 10.1016/j.plaphy.2019.04.017. Epub 2019 Apr 16. PMID: 31015091.
  15. Rufián JS, Rueda-Blanco J, Beuzón CR, Ruiz-Albert J. Protocol: an improved method to quantify activation of systemic acquired resistance (SAR). Plant Methods. 2019 Feb 14;15:16. doi: 10.1186/s13007-019-0400-5. PMID: 30809268; PMCID: PMC6374889.
  16. Castro PH, Santos MÂ, Freitas S, Cana-Quijada P, Lourenço T, Rodrigues MAA, Fonseca F, Ruiz-Albert J, Azevedo JE, Tavares RM, Castillo AG, Bejarano ER, Azevedo H. Arabidopsis thaliana SPF1 and SPF2 are nuclear-located ULP2-like SUMO proteases that act downstream of SIZ1 in plant development. J Exp Bot. 2018 Aug 31;69(19):4633-4649. doi: 10.1093/jxb/ery265. PMID: 30053161; PMCID: PMC6117582.
  17. Arroyo-Mateos M, Sabarit B, Maio F, Sánchez-Durán MA, Rosas-Díaz T, Prins M, Ruiz-Albert J, Luna AP, van den Burg HA, Bejarano ER. Geminivirus Replication Protein Impairs SUMO Conjugation of Proliferating Cellular Nuclear Antigen at Two Acceptor Sites. J Virol. 2018 Aug 29;92(18):e00611-18. doi: 10.1128/JVI.00611-18. PMID: 29950424; PMCID: PMC6146701.
  18. Belaj A, de la Rosa R, Lorite IJ, Mariotti R, Cultrera NGM, Beuzón CR, González-Plaza JJ, Muñoz-Mérida A, Trelles O, Baldoni L. Usefulness of a New Large Set of High Throughput EST-SNP Markers as a Tool for Olive Germplasm Collection Management. Front Plant Sci. 2018 Sep 21;9:1320. doi: 10.3389/fpls.2018.01320. PMID: 30298075; PMCID: PMC6160578.
  19. Rufián JS, Lucía A, Rueda-Blanco J, Zumaquero A, Guevara CM, Ortiz-Martín I, Ruiz-Aldea G, Macho AP, Beuzón CR, Ruiz-Albert J. Suppression of HopZ Effector-Triggered Plant Immunity in a Natural Pathosystem. Front Plant Sci. 2018 Aug 14;9:977. doi: 10.3389/fpls.2018.00977. PMID: 30154802; PMCID: PMC6103241.
  20. Charova SN, Gazi AD, Mylonas E, Pozidis C, Sabarit B, Anagnostou D, Psatha K, Aivaliotis M, Beuzon CR, Panopoulos NJ, Kokkinidis M. Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion. mBio. 2018 Jul 31;9(4):e01096-18. doi: 10.1128/mBio.01096-18. PMID: 30065089; PMCID: PMC6069116.
  21. Rufián JS, López-Márquez D, López-Pagán N, Grant M, Ruiz-Albert J, Beuzón CR. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae. Methods Mol Biol. 2018;1734:183-199. doi: 10.1007/978-1-4939-7604-1_15. PMID: 29288455.
  22. Rufián JS, Macho AP, Corry DS, Mansfield JW, Ruiz-Albert J, Arnold DL, Beuzón CR. Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains. Mol Plant Pathol. 2018 Mar;19(3):537-551. doi: 10.1111/mpp.12539. Epub 2017 Apr 11. PMID: 28120374; PMCID: PMC6638015.
  23. Castro PH, Couto D, Freitas S, Verde N, Macho AP, Huguet S, Botella MA, Ruiz-Albert J, Tavares RM, Bejarano ER, Azevedo H. SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana. Plant Mol Biol. 2016 Sep;92(1-2):143-59. doi: 10.1007/s11103-016-0500-9. Epub 2016 Jun 21. PMID: 27325215.
  24. Rufián JS, Sánchez-Romero MA, López-Márquez D, Macho AP, Mansfield JW, Arnold DL, Ruiz-Albert J, Casadesús J, Beuzón CR. Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host. Environ Microbiol. 2016 Oct;18(10):3593-3605. doi: 10.1111/1462-2920.13497. Epub 2016 Sep 9. PMID: 27516206.
  25. Calvo-Polanco M, Sánchez-Castro I, Cantos M, García JL, Azcón R, Ruiz-Lozano JM, Beuzón CR, Aroca R. Effects of different arbuscular mycorrhizal fungal backgrounds and soils on olive plants growth and water relation properties under well-watered and drought conditions. Plant Cell Environ. 2016 Nov;39(11):2498-2514. doi: 10.1111/pce.12807. Epub 2016 Oct 3. PMID: 27448529.
  26. Rosas-Díaz T, Macho AP, Beuzón CR, Lozano-Durán R, Bejarano ER. The C2 Protein from the Geminivirus Tomato Yellow Leaf Curl Sardinia Virus Decreases Sensitivity to Jasmonates and Suppresses Jasmonate-Mediated Defences. Plants (Basel). 2016 Jan 15;5(1):8. doi: 10.3390/plants5010008. PMID: 27135228; PMCID: PMC4844413.
  27. González-Plaza JJ, Ortiz-Martín I, Muñoz-Mérida A, García-López C, Sánchez-Sevilla JF, Luque F, Trelles O, Bejarano ER, De La Rosa R, Valpuesta V, Beuzón CR. Transcriptomic Analysis Using Olive Varieties and Breeding Progenies Identifies Candidate Genes Involved in Plant Architecture. Front Plant Sci. 2016 Mar 2;7:240. doi: 10.3389/fpls.2016.00240. PMID: 26973682; PMCID: PMC4773642.
  28. Aussel L, Beuzón CR, Cascales E. Meeting report: Adaptation and communication of bacterial pathogens. Virulence. 2016 May 18;7(4):481-90. doi: 10.1080/21505594.2016.1152441. Epub 2016 Feb 18. PMID: 26890494; PMCID: PMC4871651.
  29. Macho AP, Rufián JS, Ruiz-Albert J, Beuzón CR. Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae – Plant Interactions. Methods Mol Biol. 2016;1363:209-17. doi: 10.1007/978-1-4939-3115-6_17. PMID: 26577792.
  30. Rufián JS, Lucía A, Macho AP, Orozco-Navarrete B, Arroyo-Mateos M, Bejarano ER, Beuzón CR, Ruiz-Albert J. Auto-acetylation on K289 is not essential for HopZ1a-mediated plant defense suppression. Front Microbiol. 2015 Jul 8;6:684. doi: 10.3389/fmicb.2015.00684. PMID: 26217317; PMCID: PMC4495678.
  31. Muñoz-Mérida A, González-Plaza JJ, Cañada A, Blanco AM, García-López Mdel C, Rodríguez JM, Pedrola L, Sicardo MD, Hernández ML, De la Rosa R, Belaj A, Gil-Borja M, Luque F, Martínez-Rivas JM, Pisano DG, Trelles O, Valpuesta V, Beuzón CR. De novo assembly and functional annotation of the olive (Olea europaea) transcriptome. DNA Res. 2013 Feb;20(1):93-108. doi: 10.1093/dnares/dss036. Epub 2013 Jan 7. PMID: 23297299; PMCID: PMC3576661.
  32. Leba LJ, Cheval C, Ortiz-Martín I, Ranty B, Beuzón CR, Galaud JP, Aldon D. CML9, an Arabidopsis calmodulin-like protein, contributes to plant innate immunity through a flagellin-dependent signalling pathway. Plant J. 2012 Sep;71(6):976-89. doi: 10.1111/j.1365-313X.2012.05045.x. Epub 2012 Jun 28. PMID: 22563930.
  33. Macho AP, Zumaquero A, Gonzalez-Plaza JJ, Ortiz-Martín I, Rufián JS, Beuzón CR. Genetic analysis of the individual contribution to virulence of the type III effector inventory of Pseudomonas syringae pv. phaseolicola. PLoS One. 2012;7(4):e35871. doi: 10.1371/journal.pone.0035871. Epub 2012 Apr 27. PMID: 22558247; PMCID: PMC3338808.
  34. Sánchez-Durán MA, Dallas MB, Ascencio-Ibañez JT, Reyes MI, Arroyo-Mateos M, Ruiz-Albert J, Hanley-Bowdoin L, Bejarano ER. Interaction between geminivirus replication protein and the SUMO-conjugating enzyme is required for viral infection. J Virol. 2011 Oct;85(19):9789-800. doi: 10.1128/JVI.02566-10. Epub 2011 Jul 20. PMID: 21775461; PMCID: PMC3196459.
  35. Macho AP, Beuzón CR. Insights into plant immunity signalling: the bacterial competitive index angle. Plant Signal Behav. 2010 Dec;5(12):1590-3. doi: 10.4161/psb.5.12.13843. Epub 2010 Dec 1. PMID: 21150288; PMCID: PMC3115109.
  36. Macho AP, Guidot A, Barberis P, Beuzón CR, Genin S. A competitive index assay identifies several Ralstonia solanacearum type III effector mutant strains with reduced fitness in host plants. Mol Plant Microbe Interact. 2010 Sep;23(9):1197-205. doi: 10.1094/MPMI-23-9-1197. PMID: 20687809.
  37. Macho AP, Guevara CM, Tornero P, Ruiz-Albert J, Beuzón CR. The Pseudomonas syringae effector protein HopZ1a suppresses effector-triggered immunity. New Phytol. 2010 Sep;187(4):1018-1033. doi: 10.1111/j.1469-8137.2010.03381.x. Epub 2010 Jul 15. PMID: 20636323.
  38. Zumaquero A, Macho AP, Rufián JS, Beuzón CR. Analysis of the role of the type III effector inventory of Pseudomonas syringae pv. phaseolicola 1448a in interaction with the plant. J Bacteriol. 2010 Sep;192(17):4474-88. doi: 10.1128/JB.00260-10. Epub 2010 Jul 2. PMID: 20601478; PMCID: PMC2937392.
  39. Ortiz-Martín I, Thwaites R, Mansfield JW, Beuzón CR. Negative regulation of the Hrp type III secretion system in Pseudomonas syringae pv. phaseolicola. Mol Plant Microbe Interact. 2010 May;23(5):682-701. doi: 10.1094/MPMI-23-5-0682. PMID: 20367475.
  40. Ortiz-Martín I, Thwaites R, Macho AP, Mansfield JW, Beuzón CR. Positive regulation of the Hrp type III secretion system in Pseudomonas syringae pv. phaseolicola. Mol Plant Microbe Interact. 2010 May;23(5):665-81. doi: 10.1094/MPMI-23-5-0665. PMID: 20367474.
  41. Macho AP, Ruiz-Albert J, Tornero P, Beuzón CR. Identification of new type III effectors and analysis of the plant response by competitive index. Mol Plant Pathol. 2009 Jan;10(1):69-80. doi: 10.1111/j.1364-3703.2008.00511.x. PMID: 19161354; PMCID: PMC6640233.
  42. Rodríguez-Moreno L, Pineda M, Soukupová J, Macho AP, Beuzón CR, Barón M, Ramos C. Early detection of bean infection by Pseudomonas syringae in asymptomatic leaf areas using chlorophyll fluorescence imaging. Photosynth Res. 2008 Apr;96(1):27-35. doi: 10.1007/s11120-007-9278-6. Epub 2007 Nov 14. PMID: 18000760.
  43. Macho AP, Zumaquero A, Ortiz-Martín I, Beuzón CR. Competitive index in mixed infections: a sensitive and accurate assay for the genetic analysis of Pseudomonas syringae-plant interactions. Mol Plant Pathol. 2007 Jul;8(4):437-50. doi: 10.1111/j.1364-3703.2007.00404.x. PMID: 20507512.
  44. Ortiz-Martín I, Macho AP, Lambersten L, Ramos C, Beuzón CR. Suicide vectors for antibiotic marker exchange and rapid generation of multiple knockout mutants by allelic exchange in Gram-negative bacteria. J Microbiol Methods. 2006 Dec;67(3):395-407. doi: 10.1016/j.mimet.2006.04.011. Epub 2006 Jun 5. PMID: 16750581.